Gibberellins in Immature Seeds of Canavalia
نویسندگان
چکیده
منابع مشابه
Variations in endogenous gibberellins in developing bean seeds I. Occurrence of neutral and acidic substances.
Activities of separated and chromatographed substances in the nonacidic, acidic ethyl acetate and acidic butanol fractions from bean seeds, Phaseolus vulgaris L., cv. Bountiful and Kentucky Wonder, were measured in the Progress No. 9 dwarf pea bioassay grown under red light. Activity in the nonacidic fraction was shown to be attributable only to neutral substances and was free of acidic gibbere...
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Plant lectins, especially those purified from species of the Leguminosae family, represent the best-studied group of carbohydrate-binding proteins. Lectins purified from seeds of the Diocleinae subtribe exhibit a high degree of sequence identity notwithstanding that they show very distinct biological activities. Two main factors have been related to this feature: variance in key residues influe...
متن کاملCrystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds.
A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 A resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P2(1)2(1)2, wi...
متن کاملCrystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seeds.
The seed lectin from Canavalia gladiata was purified and crystallized. Orthorhombic crystals belonging to space group C222(1) grew within three weeks at 293 K using the hanging-drop vapour-diffusion method. Using synchrotron X-ray radiation, a complete structural data set was collected at 2.3 A resolution. The preliminary crystal structure of the lectin, determined by molecular replacement, had...
متن کاملPurification and Properties of Urease Derived from Hydrated Seeds of Jack Bean, Canavalia ensiformis (L) DC.
Urease from jack bean meal and hydrated seeds has been obtained in 25 to 33% yield with specific activity in the range of 1000 to 1070 units/mg protein. A purification of 100 to 130-fold was achieved from meal and fully soaked seeds. Use of beta-mercaptoethanol and EDTA was found essential to obtain this high yield and purity. Amino acid analysis showed all 18 amino acids commonly found in prot...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1969
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.33.592